ATS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Personal Folders
Right arrow Download to citation manager
Right arrow Author home page(s):
Toru Bando
Hiromi Wada
Right arrow Permission Requests
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chen, F.
Right arrow Articles by Fukuse, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, F.
Right arrow Articles by Fukuse, T.
Related Collections
Right arrow Lung - transplantation

Ann Thorac Surg 2006;82:465-471
© 2006 The Society of Thoracic Surgeons

Original Articles: General Thoracic

Protective Effect of a Nebulized ß2-Adrenoreceptor Agonist in Warm Ischemic–Reperfused Rat Lungs

Fengshi Chen, MDa, Takayuki Nakamura, MDb, Takuji Fujinaga, MDa, Jitian Zhang, MDa, Hiroshi Hamakawa, MDa, Mitsugu Omasa, MDa, Hiroaki Sakai, MDa, Nobuharu Hanaoka, MDa, Toru Bando, MDa, Hiromi Wada, MDa,*, Tatsuo Fukuse, MDa

a Department of Thoracic Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan
b Department of Experimental Therapeutic, Translational Research Center, Kyoto University Hospital, Kyoto, Japan

Accepted for publication January 3, 2006.

* Address correspondence to Dr Wada, Department of Thoracic Surgery, Graduate School of Medicine, Kyoto University, 54 Shogoin, Kyoto 606-8507, Japan. (Email: wadah{at}kuhp.kyoto-u.ac.jp).

BACKGROUND: It seems inevitable that non–beating-heart donors will be utilized to resolve the shortage of donors for clinical lung transplantation. The control of warm ischemia-reperfusion injury is crucial in manipulating non–beating-heart donors. We hypothesized that nebulization of a ß2-adrenoreceptor agonist, salmeterol xinafoate (SLM), during warm ischemia would increase lung tissue cyclic adenosine monophosphate (cAMP) levels, resulting in lung protection.

METHODS: Two studies were conducted. The first investigated the effect of SLM nebulization during ischemia on pulmonary ischemia-reperfusion injury, using an isolated rat lung-perfusion model. The heart-lung block was excised with cannulation of the pulmonary artery and vein, exposed to 55 minutes of ischemia at 37°C, and subsequently reperfused for 60 minutes. Several parameters were measured during reperfusion. In the second study, to measure changes in lung tissue cAMP levels during warm ischemia with or without SLM nebulization, rat lungs were harvested and exposed to 60 minutes of warm ischemia with ventilation.

RESULTS: Salmeterol xinafoate nebulization significantly decreased the pulmonary shunt fraction, airway resistance, and pulmonary vascular resistance. It also inhibited pulmonary edema throughout the reperfusion period. Lung tissue cAMP was effectively maintained by SLM nebulization at the end of reperfusion. Myeloperoxidase activity in the lungs was decreased significantly by SLM nebulization. Lung tissue cAMP levels decreased during the 60 minutes of warm ischemia, but increased with SLM nebulization (p < 0.01).

CONCLUSIONS: Our results confirmed that SLM nebulization during warm ischemia maintained lung tissue cAMP levels, resulting in the alleviation of pulmonary warm ischemia-reperfusion injury.




This article has been cited by other articles:


Home page
 ICVTSHome page
F. Chen, N. Kondo, M. Sonobe, T. Fujinaga, H. Wada, and T. Bando
Expression of endothelial cell-specific adhesion molecules in lungs after cardiac arrest
Interactive CardioVascular and Thoracic Surgery, June 1, 2008; 7(3): 437 - 440.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
P. A. Corris and J. D. Christie
Update in Transplantation 2006
Am. J. Respir. Crit. Care Med., March 1, 2007; 175(5): 432 - 435.
[Full Text] [PDF]

Home page
 Ann. Thorac. Surg.Home page
A. S. Shah and J. V. Conte
Invited commentary
Ann. Thorac. Surg., August 1, 2006; 82(2): 471 - 471.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ANN THORAC SURG ASIAN CARDIOVASC THORAC ANN EUR J CARDIOTHORAC SURG
J THORAC CARDIOVASC SURG ICVTS ALL CTSNet JOURNALS
Copyright © 2006 by The Society of Thoracic Surgeons.