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Ann Thorac Surg 2004;77:1944-1950
© 2004 The Society of Thoracic Surgeons

Original article: general thoracic

Alteration of cellular electrophysiologic properties in porcine pulmonary microcirculation after preservation with University of Wisconsin and Euro-Collins solutions

Rong-Zhen Zhang, MDa, Qin Yang, MD, PhDa, Anthony P. C. Yim, MDa, Guo-Wei He, MD, PhDa,b*

a Department of Surgery, The Chinese University of Hong Kong, Hong Kong SAR, China
b Providence Heart Institute, Albert Starr Academic Center, Department of Surgery, Oregon Health and Science University, Portland, Oregon, and Wuhan Heart Institute, The Central Hospital, Wuhan, China

Accepted for publication November 7, 2003.

* Address reprint requests to Prof He, Department of Surgery, The Chinese University of Hong Kong, Block B, 5A, Prince of Wales Hospital, Shatin, NT, Hong Kong SAR, China
e-mail: gwhe{at}cuhk.edu.hk

BACKGROUND: The effect of cold storage of porcine pulmonary microvessels in University of Wisconsin (UW) and Euro-Collins (EC) solutions on the cellular electrophysiologic properties remains unknown.

METHODS: The pulmonary microarteries (PA, 381.6 ± 62.8 µm; n = 60) and microveins (PV, 360.8 ± 54.5 µm; n = 60) were incubated with Krebs (control), UW, or EC solution at 4°C for 4 hours in a myograph. The resting membrane potential and the endothelium-derived hyperpolarizing factor–mediated hyperpolarization to bradykinin (0.1 µmol/L) in the presence of inhibitors of nitric oxide and prostacyclin, N{omega}-nitro-l-arginine, hemoglobin, and indomethacin, in a single smooth muscle cell were directly measured.

RESULTS: The resting membrane potential (–60.8 ± 1.3 mV in PA and –48.1 ± 0.7 mV in PV, n = 6) was depolarized after exposure to UW solution (to –18.4 ± 0.7 mV in PA and –13.6 ± 0.8 mV in PV; n = 8; p < 0.001). The amplitude of endothelium-derived hyperpolarizing factor–mediated hyperpolarization to bradykinin was also decreased (from 7.4 ± 0.7 mV to 2.6 ± 0.7 mV in PA and from 4.6 ± 0.5 mV to 0.9 ± 0.4 mV in PV; p < 0.001). In comparison, EC depolarized the membrane potential to a lesser extent (to –28.3 ± 0.9 mV in PA and to –21.3 ± 0.8 mV in PV; n = 8; p < 0.001) and almost abolished the hyperpolarization to bradykinin. After washout, hyperpolarization was partially restored (UW, 4.9 ± 0.7 mV in PA and 2.0 ± 0.3 mV in PV. p < 0.01; EC, 2.3 ± 0.5 mV in PA and 1.0 ± 0.3 mV in PV. p < 0.01).

CONCLUSIONS: Cold storage of porcine PA and PV with UW or EC solution impairs the electrophysiologic properties (hyperpolarization) related to endothelium–smooth muscle interaction. The alteration is more profound with EC than UW solution and in veins than in arteries. The findings urge further studies on lung preservation solutions.






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