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Ann Thorac Surg 2003;76:681-688
© 2003 The Society of Thoracic Surgeons

Original article: cardiovascular

Evidence for an altered balance between matrix metalloproteinase-9 and its inhibitors in calcific aortic stenosis

Jari Satta, MD, PhDa*, Jani Oiva, MSa, Tuula Salo, MD, PhDb, Heidi Eriksen, MDc, Pasi Ohtonen, MSa, Fausto Biancari, MD, PhDa, Tatu S. Juvonen, MD, PhDa, Ylermi Soini, MD, PhDd

a Departments of Cardiothoracic Surgery, Oulu, Finland
b Diagnostics and Oral Medicine, Institute of Dentistry, Oulu, Finland
c Clinical Chemistry,, Oulu, Finland
d Pathology, University of Oulu and Oulu University Hospital, Oulu, Finland

Accepted for publication March 10, 2003.

* Address reprint requests to Dr Satta, Department of Cardiothoracic Surgery, PO Box 5000, 90014 University of Oulu, Oulu, Finland
e-mail: jari.satta{at}oulu.fi

BACKGROUND: Recently, aortic valve stenosis has been demonstrated to exhibit increased expression of certain matrix metalloproteinases (MMPs), and this has relevantly raised the question about possible interdependency between these and their tissue inhibitors. We sought to assess the expression of elastolytic MMPs and their inhibitors (TIMPs) in nonrheumatic aortic stenosis.

METHODS: The study comprised 30 stenotic and six noncalcified human aortic valves. To measure the expression levels and the amount and molecular forms of gelatinases (MMP-2, MMP-9) and TIMPs (1, 2), in situ hybridization, gelatin zymography, and reverse zymography were carried out. Antielastin staining by a monoclonal BA-4 antibody was performed to investigate the changes of one of the main substrates of these MMPs, and to substantiate the nature of the putative MMP- synthesizing cell. The cases were also immunostained with an antibody to {alpha}-smooth muscle actin. Inflammatory cell characterization was managed by monoclonal mouse antibodies (UCHL-1, L26, and PGM-1).

RESULTS: Compared with the controls, the calcific valves showed increased mRNA expression and activation of MMP-9, and this was associated with typical characteristics of valve disease. MMP-2 mRNA production was rare, but proMMP-2 protein was detected in all valves. In agreement with the interdependency between MMP-9 and its inhibitors, a suggestive imbalance came out in diseased valves.

CONCLUSIONS: The disproportion between MMP-9 and its tissue inhibitors may favor a persistent MMP activation state within the calcific valve and likely contribute to the valvular remodeling process in the setting of developing aortic stenosis.




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