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Michael R. Phillips
Hiroki Yamaguchi
James J. Morris
Hartzell V. Schaff
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Ann Thorac Surg 1998;66:1191-1197
© 1998 The Society of Thoracic Surgeons

Original articles: cardiovascular

Endothelial sodding of the permaflow prosthetic coronary artery bypass conduit

Michael R. Phillips, MDa, Hiroki Yamaguchi, MDa, Virginia M. Miller, PhDb, Stuart Williams, PhDc, James J. Morris, MDa, Hartzell V. Schaff, MDa

a Division of Thoracic and Cardiovascular Surgery , Mayo Clinic and Mayo Foundation, Rochester, Minnesota, USA
b Department of Physiology and Biophysics, Mayo Clinic and Mayo Foundation, Rochester, Minnesota USA
c Department of Surgery Research, University of Arizona, Tucson, Arizona USA

Address reprint requests to Dr Schaff, Mayo Clinic, 200 First St SW, Rochester, MN 55905

Presented at the Poster Session of the Thirty-fourth Annual Meeting of The Society of Thoracic Surgeons, New Orleans, LA, Jan 26–28, 1998.

Background. Experiments were designed to determine the feasibility of sodding an endothelial monolayer within the lumen of a prosthetic conduit applied to the canine coronary circulation.

Methods. Autologous endothelial cells were sodded onto the luminal surface of the Permaflow conduit and immediately implanted to bypass the left circumflex coronary artery in adult mongrel dogs (n = 9). Unsodded Permaflow conduits were implanted as controls (n = 8). At 3 weeks, grafts were explanted and examined by scanning electron microscopy and immunostained for canine von Willebrand factor.

Results. Sodded grafts contained a confluent endothelial cell layer devoid of adherent thrombus or platelets and stained positively for canine von Willebrand factor. Unsodded grafts contained no endothelium and retained adherent platelets, collagen, and fibrin. Effluent from sodded grafts stimulated with calcium ionophore A23187 caused a significantly greater relaxation of its bioassay ring than effluent from unsodded grafts (60% ± 21% versus 12% ± 5%; n = 8, p < 0.03).

Conclusions. Sodding of endothelial cells onto a Permaflow coronary artery bypass graft results in a confluent, viable, nonthrombogenic, endothelial monolayer and releases vasodilator substances in response to calcium ionophore A23187. Endothelial sodding may optimize prosthetic grafts.






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Copyright © 1998 by The Society of Thoracic Surgeons.