ATS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Personal Folders
Right arrow Download to citation manager
Right arrow Author home page(s):
Ryosei Kuribayashi
Right arrow Permission Requests
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chanda, J.
Right arrow Articles by Shibata, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chanda, J.
Right arrow Articles by Shibata, Y.

Ann Thorac Surg 1998;66:449-454
© 1998 The Society of Thoracic Surgeons

Original articles: cardiovascular

Inhibitory effect of photooxidation on intimal and medial thickening of saphenous vein

Jyotirmay Chanda, MDa, Ryosei Kuribayashi, MDa, Ke-Xiang Liu, MDa, Yoshiki Shibata, MDa

a Department of Cardiovascular Surgery, Akita University School of Medicine, Akita, Japan

Accepted for publication March 16, 1998.

Address reprint requests to Dr Chanda, Bejpara, Sreedhar Tank Rd, Jessore 7400, Bangladesh
e-mail: (eximpak{at}bdmail.net [Attention: Dr J Chanda])

Background. The inhibitory effect of short-term photooxidation on medial and neointimal proliferation of human saphenous vein was investigated.

Methods. Culture medium–filled surgically prepared saphenous vein segments were photooxidized in 0.01% methylene blue solution for 5 minutes. Photooxidized and nonphotooxidized saphenous veins were checked for viability of endothelial cells by culturing vein segments for 21 days followed by histologic and immunohistochemical studies.

Results. Endothelial cells of saphenous vein segments remained unaffected after photooxidation. Both the intima and media of nonphotooxidized veins became highly cellular and thickened because of the proliferation and migration of smooth muscle cells. Like precultured fresh saphenous vein, intimal (0.031 ± 0.017 mm; p = 0.0067) and medial thicknesses (0.702 ± 0.123 mm; p < 0.0001) and proliferating cell nuclear antigen–positive cell count (14 ± 8/mm2; p = 0.0005) of cultured photooxidized veins were significantly less than those of cultured nonphotooxidized veins (intimal thickness, 0.059 ± 0.041 mm; medial thickness, 0.997 ± 0.228 mm; proliferating cell nuclear antigen positive cell count, 34 ± 16/mm2.

Conclusions. Methylene blue–induced short-term photooxidation is effective in inhibition of intimal and medial thickening of saphenous vein.




This article has been cited by other articles:


Home page
 Ann. Thorac. Surg.Home page
K.-X. Liu, F. Yamamoto, S. Sekine, Y. Goto, K. Seki, K. Kondoh, and Y. Fu
Inhibitory effect of methylene blue-induced photooxidation on intimal thickening of vein graft
Ann. Thorac. Surg., July 1, 1999; 68(1): 84 - 88.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ANN THORAC SURG ASIAN CARDIOVASC THORAC ANN EUR J CARDIOTHORAC SURG
J THORAC CARDIOVASC SURG ICVTS ALL CTSNet JOURNALS
Copyright © 1998 by The Society of Thoracic Surgeons.