ATS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Personal Folders
Right arrow Download to citation manager
Right arrow Author home page(s):
Hiromi Wada
Right arrow Permission Requests
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Isowa, N.
Right arrow Articles by Wada, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Isowa, N.
Right arrow Articles by Wada, H.

Ann Thorac Surg 1996;61:542-545
© 1996 The Society of Thoracic Surgeons

Original Article: General Thoracic

Trehalose-Containing Solutions Enhance Preservation of Cultured Endothelial Cells

Noritaka Isowa, MD, Shigeki Hitomi, MD, Hiromi Wada, MD

Department of Thoracic Surgery, Chest Disease Research Institute, Kyoto University, Kyoto, Japan

Accepted for publication October 25, 1995.

Background and Methods. We have developed two types of preservation solutions containing trehalose. ET-Kyoto solution (ET-K) is an extracellular type and IT-Kyoto solution (IT-K) is an intracellular type. In the present study we examined with in vitro assays the ability of ET-K, IT-K, Euro-Collins (EC), and University of Wisconsin (UW) solutions to preserve a murine endothelial cell line. The viability of cells stored in the solutions at 4°C was determined by trypan blue exclusion and MTT assay.

Results. Trypan blue exclusion showed the viability after 48 hours of cold storage to be 49.5 ± 4.7% (mean ± standard error) in ET-K, 59.5 ± 0.7% in IT-K, 29.2 ± 2.5% in EC, and 55.3 ± 7.6% in UW (ET-K or UW versus EC, p < 0.05; IT-K versus EC, p < 0.01). MTT assay absorbance values for cells after 48 hours of cold storage were 0.366 ± 0.0066 (mean ± standard error) in ET-K, 0.358 ± 0.0044 in IT-K, 0.336 ± 0.011 in EC, and 0.362 ± 0.0019 in UW (ET-K or UW versus EC, p < 0.05). After 120 hours, absorbance values for cells were 0.303 ± 0.0038 in ET-K, 0.269 ± 0.0034 in IT-K, 0.186 ± 0.011 in EC, and 0.265 ± 0.0066 in UW (ET-K versus UW, p < 0.05; ET-K versus IT-K, p < 0.01; ET-K, IT-K or UW versus EC, p < 0.01).

Conclusions. As far as the ability to preserve a murine endothelial cell line at a low temperature was concerned, the ET-K solution was superior to the UW solution, the IT-K solution and UW solution were equal, and the ET-K and IT-K solutions were superior to the EC solution.




This article has been cited by other articles:


Home page
 Ann. Thorac. Surg.Home page
A. Parolari, P. Rubini, A. Cannata, L. Bonati, F. Alamanni, E. Tremoli, and P. Biglioli
Endothelial damage during myocardial preservation and storage
Ann. Thorac. Surg., February 1, 2002; 73(2): 682 - 690.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. A. Cardella, S. Keshavjee, E. Mourgeon, S. D. Cassivi, S. Fischer, N. Isowa, A. Slutsky, and M. Liu
A novel cell culture model for studying ischemia-reperfusion injury in lung transplantation
J Appl Physiol, October 1, 2000; 89(4): 1553 - 1560.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. KAWASHIMA, T. BANDO, T. NAKAMURA, N. ISOWA, M. LIU, S. TOYOKUNI, S. HITOMI, and H. WADA
Cytoprotective Effects of Nitroglycerin in Ischemia-Reperfusion-Induced Lung Injury
Am. J. Respir. Crit. Care Med., March 1, 2000; 161(3): 935 - 943.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ANN THORAC SURG ASIAN CARDIOVASC THORAC ANN EUR J CARDIOTHORAC SURG
J THORAC CARDIOVASC SURG ICVTS ALL CTSNet JOURNALS
Copyright © 1996 by The Society of Thoracic Surgeons.