HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Author home page(s): Mehmet C. Oz Eric A. Rose Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rafii, S. Articles by Levin, H. R. Search for Related Content PubMed PubMed Citation Articles by Rafii, S. Articles by Levin, H. R. Related Collections Related Article

Ann Thorac Surg 1995;60:1627-1632
© 1995 The Society of Thoracic Surgeons

---

Original Articles: Cardiovascular

Characterization of Hematopoietic Cells Arising on the Textured Surface of Left Ventricular Assist Devices

Department of Hematology-Oncology, Cornell University Medical Center, and Divisions of Cardiothoracic Surgery and Circulatory Physiology, College of Physicians & Surgeons, Columbia University, New York, New York

Background. Textured biomaterial surfaces in implantable left ventricular assist devices induce development of a nonthrombotic neointimal surface and allow elimination of anticoagulation therapy in device recipients. Characterization of the hematopoietic cells formed within the neointimal surfaces of these devices will contribute to our understanding of this unique neointima.

Methods. The blood-contacting surface of seven ThermoCardiosystems left ventricular assist devices was removed, washed with phosphate-buffered saline solution, and digested with 0.1% collagenase for 15 to 20 minutes. The hematopoietic cells released from the explants were isolated and analyzed by flow cytometry and immunohistochemical staining.

Results. More than 80% ± 6% of hematopoietic cells isolated in this fashion are of myelomonocytic origin and express CD14, CD15, and CD33 surface molecules. Four percent of cells express the CD34 surface marker, which suggests that the neointima is colonized by pluripotent hematopoietic stem cells. Continuous culture of these hematopoietic cells in the presence of the cytokines interleukin-3, c-kit ligand, granulocyte colony-stimulating factor, and granulocyte/macrophage colony-stimulating factor resulted in tenfold expansion by day 7 and 25-fold expansion by day 14.

Conclusions. Pluripotent hematopoietic cells with a high proliferative capacity colonize textured surfaces of left ventricular assist devices and may contribute to the development of a biologically nonthrombogenic neointima.

Related Article

Cellular Linings of Ventricular Assist Devices

Timothy Scott-Burden and O. H. Frazier
Ann. Thorac. Surg. 1995 60: 1561-1562. [Extract] [Full Text]

This article has been cited by other articles:

				S. G. Drakos, J. C. Stringham, J. W. Long, E. M. Gilbert, T. C. Fuller, B. K. Campbell, B. D. Horne, M. E. Hagan, K. E. Nelson, J. M. Lindblom, et al. Prevalence and risks of allosensitization in HeartMate left ventricular assist device recipients: The impact of leukofiltered cellular blood product transfusions J. Thorac. Cardiovasc. Surg., June 1, 2007; 133(6): 1612 - 1619. [Abstract] [Full Text] [PDF]

				S. G. Drakos, A. G. Kfoury, J. W. Long, J. C. Stringham, T. C. Fuller, K. E. Nelson, B. K. Campbell, E. M. Gilbert, and D. G. Renlund Low-dose prophylactic intravenous immunoglobulin does not prevent HLA sensitization in left ventricular assist device recipients. Ann. Thorac. Surg., September 1, 2006; 82(3): 889 - 893. [Abstract] [Full Text] [PDF]

				F. W. Sellke, R. Laham, E. J. Suuronen, and M. Ruel Angiogenesis for the treatment of inoperable coronary disease: the future. Seminars in Cardiothoracic and Vascular Anesthesia, June 1, 2006; 10(2): 184 - 188. [Abstract] [PDF]

				K. Naruse, Y. Hamada, E. Nakashima, K. Kato, R. Mizubayashi, H. Kamiya, Y. Yuzawa, S. Matsuo, T. Murohara, T. Matsubara, et al. Therapeutic Neovascularization Using Cord Blood-Derived Endothelial Progenitor Cells for Diabetic Neuropathy Diabetes, June 1, 2005; 54(6): 1823 - 1828. [Abstract] [Full Text] [PDF]

				C. Dong, L. E. Crawford, and P. J. Goldschmidt-Clermont Endothelial Progenitor Obsolescence and Atherosclerotic Inflammation J. Am. Coll. Cardiol., May 3, 2005; 45(9): 1458 - 1460. [Full Text] [PDF]

				G. S. Kumpati, D. J. Cook, E. H. Blackstone, J. Rajeswaran, A. S. Abdo, J. B. Young, R. C. Starling, N. G. Smedira, and P. M. McCarthy HLA sensitization in ventricular assist device recipients: Does type of device make a difference? J. Thorac. Cardiovasc. Surg., June 1, 2004; 127(6): 1800 - 1807. [Abstract] [Full Text] [PDF]

				S Francis Endothelial progenitor cells and coronary artery disease Heart, June 1, 2004; 90(6): 591 - 592. [Abstract] [Full Text] [PDF]

				F. A. Scappaticci Mechanisms and Future Directions for Angiogenesis-Based Cancer Therapies J. Clin. Oncol., September 15, 2002; 20(18): 3906 - 3927. [Abstract] [Full Text] [PDF]

				M. Gill, S. Dias, K. Hattori, M. L. Rivera, D. Hicklin, L. Witte, L. Girardi, R. Yurt, H. Himel, and S. Rafii Vascular Trauma Induces Rapid but Transient Mobilization of VEGFR2+AC133+ Endothelial Precursor Cells Circ. Res., February 2, 2001; 88(2): 167 - 174. [Abstract] [Full Text] [PDF]

				M. Peichev, A. J. Naiyer, D. Pereira, Z. Zhu, W. J. Lane, M. Williams, M. C. Oz, D. J. Hicklin, L. Witte, M. A. S. Moore, et al. Expression of VEGFR-2 and AC133 by circulating human CD34+ cells identifies a population of functional endothelial precursors Blood, February 1, 2000; 95(3): 952 - 958. [Abstract] [Full Text] [PDF]

				T. B. Spanier, J. M. Chen, M. C. Oz, D. M. Stern, E. A. Rose, and A. M. Schmidt TIME-DEPENDENT CELLULAR POPULATION OF TEXTURED-SURFACE LEFT VENTRICULAR ASSIST DEVICES CONTRIBUTES TO THE DEVELOPMENT OF A BIPHASIC SYSTEMIC PROCOAGULANT RESPONSE J. Thorac. Cardiovasc. Surg., September 1, 1999; 118(3): 404 - 413. [Abstract] [Full Text] [PDF]

				C. Baufreton, M. Kirsch, and D. Y. Loisance Reply Ann. Thorac. Surg., June 1, 1999; 67(6): 1828 - 1829. [Full Text] [PDF]

				C. Baufreton, M. Kirsch, and D. Y. Loisance Measures to control blood activation during assisted circulation Ann. Thorac. Surg., November 1, 1998; 66(5): 1837 - 1844. [Abstract] [Full Text] [PDF]

				Q. Shi, S. Rafii, M. H.-D. Wu, E. S. Wijelath, C. Yu, A. Ishida, Y. Fujita, S. Kothari, R. Mohle, L. R. Sauvage, et al. Evidence for Circulating Bone Marrow-Derived Endothelial Cells Blood, July 15, 1998; 92(2): 362 - 367. [Abstract] [Full Text] [PDF]

				J. P. Slater, E. A. Rose, H. R. Levin, O. H. Frazier, J. K. Roberts, A. D. Weinberg, and M. C. Oz Low Thromboembolic Risk Without Anticoagulation Using Advanced-Design Left Ventricular Assist Devices Ann. Thorac. Surg., November 1, 1996; 62(5): 1321 - 1327. [Abstract] [Full Text]

				T. Spanier, M. Oz, H. Levin, A. Weinberg, K. Stamatis, D. Stern, E. Rose, and A. M. Schmidt ACTIVATION OF COAGULATION AND FIBRINOLYTIC PATHWAYS IN PATIENTS WITH LEFT VENTRICULAR ASSIST DEVICES J. Thorac. Cardiovasc. Surg., October 1, 1996; 112(4): 1090 - 1097. [Abstract] [Full Text]

				E. A. Rose and D. J. Goldstein Wearable Long-Term Mechanical Support for Patients With End-Stage Heart Disease: A Tenable Goal Ann. Thorac. Surg., January 1, 1996; 61(1): 399 - 402. [Abstract] [Full Text]

				T. Scott-Burden and O. H. Frazier Cellular Linings of Ventricular Assist Devices Ann. Thorac. Surg., December 1, 1995; 60(6): 1561 - 1562. [Full Text]