HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hosoda, H. Articles by Suzuki, A. Search for Related Content PubMed PubMed Citation Articles by Hosoda, H. Articles by Suzuki, A.

The Annals of Thoracic Surgery, Vol 58, 1631-1636, Copyright © 1994 by The Society of Thoracic Surgeons

ARTICLES

Effect of pinacidil on rat hearts undergoing hypothermic cardioplegia

H Hosoda, M Sunamori and A Suzuki
Department of Thoracic-Cardiovascular Surgery, Tokyo Medical and Dental University, School of Medicine, Japan.

We studied the effect of pinacidil, a potassium-channel opener, on the hemodynamic, biochemical, and ultrastructural changes in rat hearts undergoing hypothermic cardioplegia. Fifty-four male Wistar rats weighing 250 to 300 g were used. Isolated hearts were prepared for modified Langendorff circulation in the working mode using modified Krebs-Henseleit bicarbonate solution bubbled with a 95% O2 and 5% CO2 gas mixture. Eighty minutes of cardioplegia at 25 degrees C was followed by normothermic reperfusion for 30 minutes. Pinacidil, 5, 10, or 50 mumol/L added to the cardioplegic solution, did not affect heart rate, but is significantly improved the recovery of aortic flow as compared with controls (88.1% +/- 4.3 [5 mumol/L]; 83.2% +/- 8.5% [10 mumol/L]; 90.3% +/- 5.3% [50 mumol/L] compared with 55.6 +/- 4.3% [control]; p < 0.05). Administration of pinacidil during reperfusion did not further enhance the recovery of aortic flow. The dose-response curve of aortic flow to the pinacidil concentrations was flat from 5 to 50 mumol/L. However, preservation of myocardial adenosine triphosphate and calcium concentrations and mitochondrial morphology suggested that the optimal concentration of pinacidil cardioplegia is 10 mumol/L.

This article has been cited by other articles:

				D. J. Chambers Mechanisms and alternative methods of achieving cardiac arrest Ann. Thorac. Surg., February 1, 2003; 75(2): S661 - 666. [Abstract] [Full Text] [PDF]

				N. Matsuda, K. G. Morgan, and F. W. Sellke Effects of pinacidil on coronary Ca2+-myosin phosphorylation in cold potassium cardioplegia model Am J Physiol Heart Circ Physiol, September 1, 2000; 279(3): H882 - H888. [Abstract] [Full Text] [PDF]

				J.-F. Bouchard, J. Chouinard, and D. Lamontagne Participation of prostaglandin E2 in the endothelial protective effect of ischaemic preconditioning in isolated rat heart Cardiovasc Res, January 14, 2000; 45(2): 418 - 427. [Abstract] [Full Text] [PDF]

				D. J. Chambers and D. J. Hearse Developments in cardioprotection: ""polarized"" arrest as an alternative to ""depolarized"" arrest Ann. Thorac. Surg., November 1, 1999; 68(5): 1960 - 1966. [Abstract] [Full Text] [PDF]

				M. Kirsch, C. Baufreton, C. Fernandez, S. Brunet, F. Pasteau, A. Astier, and D. Y. Loisance Preconditioning with cromakalim improves long-term myocardial preservation for heart transplantation Ann. Thorac. Surg., August 1, 1998; 66(2): 417 - 424. [Abstract] [Full Text] [PDF]

				J.-F. Bouchard and D. Lamontagne Protection afforded by preconditioning to the diabetic heart against ischaemic injury Cardiovasc Res, January 1, 1998; 37(1): 82 - 90. [Abstract] [Full Text] [PDF]

				P. Menasche, C. Mouas, and C. Grousset Is Potassium Channel Opening an Effective Form of Preconditioning Before Cardioplegia? Ann. Thorac. Surg., June 1, 1996; 61(6): 1764 - 1768. [Abstract] [Full Text]

				I. B. Krukenkamp and S. Levitsky Myocardial Protection: Modern Studies Ann. Thorac. Surg., May 1, 1996; 61(5): 1581 - 1582. [Full Text]

				P. Menasche, E. Kevelaitis, C. Mouas, C. Grousset, A. Piwnica, and G. Bloch PRECONDITIONING WITH POTASSIUM CHANNEL OPENERS:A NEW CONCEPT FOR ENHANCING CARDIOPLEGIC PROTECTION? J. Thorac. Cardiovasc. Surg., December 1, 1995; 110(6): 1606 - 1614. [Abstract] [Full Text]